Indirect enzyme-linked immunosorbent assay (ELISA)

Learning objectives

To understand the principle behind the use of the ELISA technique in screening for AIDS.
To describe the different steps invloved in the ELISA technique.
To point out the differences between direct ELISA and indirect ELISA.

ELISA, Enzyme Linked Immunosorbent Assay, is a is an immuno-enzymatic technique that enables the detection of antigen-antibody reactions thanks to a reaction with colored products. ELISA is a technique currently used in the process of screening for HIV infections.